Mouse NT-ProANP-N-Terminal Pro-Atrial Natriuretic Peptide ELISA Kit

Mouse NT-ProANP-N-Terminal Pro-Atrial Natriuretic Peptide ELISA Kit

318.00455.00

Specification 96T, 48T

SKU: ELK2401 Category: Tag:

Overview of Mouse NT-ProANP-N-Terminal Pro-Atrial Natriuretic Peptide ELISA Kit

Product name: Mouse NT-ProANP-N-Terminal Pro-Atrial Natriuretic Peptide ELISA Kit
Reactivity: Mouse
Alternative Names: NT-Pro-ANP; N-ANPNT-Pro-ANP; N-ANP
Assay Type: Sandwich
Sensitivity: 29 pg/mL
Standard: 5000 ng/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5 h
Research Area: Endocrinology;Cardiovascular biology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NT-ProANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NT-ProANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NT-ProANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NT-ProANP in the samples is then determined by comparing the OD of the samples to the standard curve.

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Size

48T, 96T, 96T,48T

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