Name: Mouse DEFa5-Defensin Alpha 5, Paneth Cell Specific ELISA Kit - Gencyst
SKU: ELK2462
Availability: InStock

Mouse DEFa5-Defensin Alpha 5, Paneth Cell Specific ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

Clear

SKU: ELK2462 Category: ELISA Kit Tag: ELISA Kit

Overview of Mouse DEFa5-Defensin Alpha 5, Paneth Cell Specific ELISA Kit

Product name:	Mouse DEFa5-Defensin Alpha 5, Paneth Cell Specific ELISA Kit
Reactivity:	Mouse
Alternative Names:	DEF-A5; DEF5; HD-5
Assay Type:	Sandwich
Sensitivity:	13.1 pg/mL
Standard:	2000 pg/mL
Detection Range:	31.25-2000 pg/mL
Sample Type:	serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5 h
Research Area:	Infection immunity;Hepatology;Gastroenterology;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse DEFa5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse DEFa5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse DEFa5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse DEFa5 in the samples is then determined by comparing the OD of the samples to the standard curve.