Cattle ALP(Alkaline Phosphatase) ELISA Kit

Cattle ALP(Alkaline Phosphatase) ELISA Kit


Specification 96T, 48T

SKU: ELK1355 Category: Tag:

Overview of Cattle ALP(Alkaline Phosphatase) ELISA Kit

Product name: Cattle ALP(Alkaline Phosphatase) ELISA Kit
Reactivity: Cattle
Alternative Names: AKP; ALKP; Basic Phosphatase
Assay Type: Sandwich
Sensitivity: 0.066 ng/mL
Standard: 10 pg/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;Tumor immunity; Infection immunity;Hepatology;Bone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle ALP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle ALP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle ALP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle ALP in the samples is then determined by comparing the OD of the samples to the standard curve.


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48T, 96T, 96T,48T

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