Guinea pig HB-Hemoglobin ELISA Kit

Guinea pig HB-Hemoglobin ELISA Kit

367.00523.00

Specification 96T, 48T

SKU: ELK1353 Category: Tag:

Overview of Guinea pig HB-Hemoglobin ELISA Kit

Product name: Guinea pig HB-Hemoglobin ELISA Kit
Reactivity: Guinea pig
Alternative Names: Hgb; Haemoglobin; Heterotetramer(αβ)2
Assay Type: Sandwich
Sensitivity: 4.58 ng/mL
Standard: 800 ng/mL
Detection Range: 12.5-800 ng/mL
Sample Type: Serum, plasma, erythrocyte lysates.
Assay Length: 3.5 h
Research Area: Metabolic pathway;Infection immunity;Hematology;Neuro science;
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guinea pig HB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig HB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guinea pig HB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guinea pig HB in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

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Additional information

Size

48T, 96T, 96T,48T

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