Name: Human ALT-Alanine Aminotransferase ELISA Kit - Gencyst
SKU: ELK2461
Availability: InStock

Human ALT-Alanine Aminotransferase ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

SKU: ELK2461 Category: ELISA Kit Tag: ELISA Kit

Overview of Human ALT-Alanine Aminotransferase ELISA Kit

Product name:	Human ALT-Alanine Aminotransferase ELISA Kit
Reactivity:	Human
Alternative Names:	GPT; ALAT; SGPT; AAT1; GPT1; Serum Glutamic Pyruvic Transaminase; Alanine Transaminase; Glutamate pyruvate transaminase 1; Glutamic--alanine transaminase 1
Assay Type:	Sandwich
Sensitivity:	0.057 U/L
Standard:	10 U/L
Detection Range:	0.16-10 U/L
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5 h
Research Area:	Enzyme & Kinase;Infection immunity;Hepatology;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ALT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ALT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ALT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ALT in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T