Human AP13-Apelin 13 ELISA Kit
Overview of Human AP13-Apelin 13 ELISA Kit
Product name: |
Human AP13-Apelin 13 ELISA Kit |
Reactivity: | Human |
Alternative Names: | APLN13 |
Assay Type: | Competitive Inhibition |
Sensitivity: | 8.24 pg/mL |
Standard: | 1600 pg/mL |
Detection Range: | 25-1600 pg/mL |
Sample Type: | Serum, plasma and other biological fluids |
Assay Length: | 2 h |
Research Area: | Metabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Gastroenterology; |
Test Principle: | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP13 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP13. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP13 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
Size | 48T, 96T |
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