Human APOB100(Apolipoprotein B100) ELISA Kit

Human APOB100(Apolipoprotein B100) ELISA Kit

262.00

Specification 96T, 48T

SKU: ELK1275 Category: Tag:

Overview Human APOB100(Apolipoprotein B100) ELISA Kit

Product name: Human APOB100(Apolipoprotein B100) ELISA Kit
Reactivity: Human
Alternative Names: Apo-B100
Assay Type: Sandwich
Sensitivity: 9.15 ng/mL
Standard: 1500 ng/mL
Detection Range: 23.44-1500 ng/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Nutrition metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APOB100. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APOB100. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Human APOB100, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a colour change. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APOB100 in the samples is then determined by comparing the OD of the samples to the standard curve.

 

Read More Description

 

Product name: Human APOB100(Apolipoprotein B100) ELISA Kit
Reactivity: Human
Alternative Names: Apo-B100
Assay Type: Sandwich
Sensitivity: 9.15 ng/mL
Standard: 1500 ng/mL
Detection Range: 23.44-1500 ng/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Nutrition metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APOB100. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APOB100. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Human APOB100, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a colour change. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APOB100 in the samples is then determined by comparing the OD of the samples to the standard curve.

 

Read More Description

Additional information

Size

48T, 96T, 96T,48T

Top rated products