Human bEP-Beta-Endorphin ELISA Kit

Human bEP-Beta-Endorphin ELISA Kit

318.00455.00

Specification 96T, 48T

SKU: ELK2405 Category: Tag:

Overview of Human bEP-Beta-Endorphin ELISA Kit

Product name: Human bEP-Beta-Endorphin ELISA Kit
Reactivity: Human
Alternative Names: Beta EP; b-EP
Assay Type: Competitive Inhibition
Sensitivity: 4.55 pg/mL
Standard: 1000 ng/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, cerebrospinal fluid and other biological fluids
Assay Length: 2 h
Research Area: Signal transduction;Endocrinology;Neuro science;Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human bEP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human bEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human bEP in the samples is then determined by comparing the OD of the samples to the standard curve.

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Additional information

Size

48T, 96T, 96T,48T

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