Human CA19-9(Carbohydrate Antigen 19-9) ELISA Kit

Human CA19-9(Carbohydrate Antigen 19-9) ELISA Kit

262.00364.00

Specification 96T, 48T

SKU: ELK1198 Category: Tag:

Overview of Human CA19-9(Carbohydrate Antigen 19-9) ELISA Kit

Product name: Human CA19-9(Carbohydrate Antigen 19-9) ELISA Kit
Reactivity: Human
Alternative Names: sialylated Lewis(a)Antigen; Cancer Antigen 19-9
Assay Type: Sandwich
Sensitivity: 0.28 U/mL
Standard: 50 U/mL
Detection Range: 0.79-50 U/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Tumor immunity;Hepatology;Gastroenterology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CA19-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA19-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Human CA19-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a colour change. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA19-9 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Size

48T, 96T, 96T,48T