Human CD30L-Cluster Of Differentiation 30 Ligand ELISA Kit
Overview of Human CD30L-Cluster Of Differentiation 30 Ligand ELISA Kit
Product name: |
Human CD30L-Cluster Of Differentiation 30 Ligand ELISA Kit |
Reactivity: | Human |
Alternative Names: | CD153; TNFSF8; CD30-L; Tumor Necrosis Factor Ligand Superfamily Member 8 |
Assay Type: | Sandwich |
Sensitivity: | 34 pg/mL |
Standard: | 5000 pg/mL |
Detection Range: | 78.13-5000 pg/mL |
Sample Type: | Serum, plasma, tissue homogenates and other biological fluids |
Assay Length: | 3.5 h |
Research Area: | Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule; |
Test Principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD30L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD30L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD30L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD30L in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
Size | 48T, 96T |
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