Name: Human COL4-Collagen Type IV ELISA Kit - Gencyst
SKU: ELK2331
Availability: InStock

Human COL4-Collagen Type IV ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

SKU: ELK2331 Category: ELISA Kit Tag: ELISA Kit

Overview of Human COL4-Collagen Type IV ELISA Kit

Product name:	Human COL4-Collagen Type IV ELISA Kit
Reactivity:	Human
Alternative Names:	Type-IV Collagen
Assay Type:	Sandwich
Sensitivity:	3.1 ng/mL
Standard:	500 ng/mL
Detection Range:	7.82-500 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5 h
Research Area:	Tumor immunity;Hepatology;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COL4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COL4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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