Human CRH-Corticotropin Releasing Hormone ELISA Kit

Human CRH-Corticotropin Releasing Hormone ELISA Kit

409.00583.00

Specification 96T, 48T

SKU: ELK2411 Category: Tag:

Overview of Human CRH-Corticotropin Releasing Hormone ELISA Kit

Product name: Human CRH-Corticotropin Releasing Hormone ELISA Kit
Reactivity: Human
Alternative Names: CRF; Corticotropin Releasing Factor; Corticoliberin
Assay Type: Competitive Inhibition
Sensitivity: 4.91 pg/mL
Standard: 1000 ng/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2 h
Research Area: Endocrinology;Cardiovascular biology;Neuro science;Hormone metabolism;Urology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human CRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CRH in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

dNTP Mix PCR Grade 10 mM each

Pfu DNA Polymerase

One Step SYBR Green RT-PCR Mix

Hot start Taq DNA Polymerase

EntiLink PCR Master Mix

Additional information

Size

48T, 96T

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