Human D2D(D-Dimer) ELISA Kit

Human D2D(D-Dimer) ELISA Kit

364.00

Specification 96T, 48T

SKU: ELK1213 Category: Tag:

Overview of Human D2D(D-Dimer) ELISA Kit

Product name: Human D2D(D-Dimer) ELISA Kit
Reactivity: Human
Alternative Names: D 2 Dimer
Assay Type: Sandwich
Sensitivity: 26.7 ng/mL
Standard: 5000 ng/mL
Detection Range: 78.13-5000 ng/mL
Sample Type: plasma
Assay Length: 3.5h
Research Area: Hematology;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human D2D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human D2D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Human D2D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a color change. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human D2D in the samples is then determined by comparing the OD of the samples to the standard curve.

 

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Product name: Human D2D(D-Dimer) ELISA Kit
Reactivity: Human
Alternative Names: D 2 Dimer
Assay Type: Sandwich
Sensitivity: 26.7 ng/mL
Standard: 5000 ng/mL
Detection Range: 78.13-5000 ng/mL
Sample Type: plasma
Assay Length: 3.5h
Research Area: Hematology;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human D2D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human D2D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Human D2D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a color change. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human D2D in the samples is then determined by comparing the OD of the samples to the standard curve.

 

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Additional information

Size

48T, 96T, 96T,48T

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