Name: Human DKK1-Dickkopf Related Protein 1 ELISA Kit - Gencyst
SKU: ELK2097
Availability: InStock

Human DKK1-Dickkopf Related Protein 1 ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

Clear

SKU: ELK2097 Category: ELISA Kit Tag: ELISA Kit

Overview of Human DKK1-Dickkopf Related Protein 1 ELISA Kit

Product name:	Human DKK1-Dickkopf Related Protein 1 ELISA Kit
Reactivity:	Human
Alternative Names:	SK; Dickkopf Homolog 1(Xenopus Laevis)
Assay Type:	Sandwich
Sensitivity:	0.056 ng/mL
Standard:	10 pg/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5 h
Research Area:	Signal transduction;Tumor immunity;Hematology;Developmental science;Bone metabolism;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DKK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DKK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DKK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DKK1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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