Human Dyn-Big Dynorphin ELISA Kit
Overview of Human Dyn-Big Dynorphin ELISA Kit
Product name: |
Human Dyn-Big Dynorphin ELISA Kit |
Reactivity: | Human |
Alternative Names: | B-DYN; Biocytin 13 Dynorphin A 1-13 Amide |
Assay Type: | Competitive Inhibition |
Sensitivity: | 2.56 pg/mL |
Standard: | 500 ng/mL |
Detection Range: | 7.82-500 pg/mL |
Sample Type: | Serum, plasma and other biological fluids |
Assay Length: | 2 h |
Research Area: | Signal transduction;Neuro science; |
Test Principle: | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Dyn protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Dyn. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Dyn in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
Size | 48T, 96T |
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