Human E-Cad-E-Cadherin ELISA Kit

Human E-Cad-E-Cadherin ELISA Kit

262.00364.00

Specification 96T, 48T

SKU: ELK1006 Category: Tag:

Overview of Human E-Cad-E-Cadherin ELISA Kit

Product name: Human E-Cad-E-Cadherin ELISA Kit
Reactivity: Human
Alternative Names: CD324; CDH1; Arc1; ECAD; CDH-E; E-CAD; LCAM; UVO; Cadherin 1 Type 1; Uvomorulin; E-Cadherin; Calcium-Dependent Adhesion Protein, Epithelial; Liver Cell Adhesion Molecule; Cadherin, Epithelial
Assay Type: Sandwich
Sensitivity: 25.1 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.5-4000 pg/mL
Sample Type: serum, plasma, urine, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: CD & Adhesion molecule;Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human E-Cad. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human E-Cad. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human E-Cad, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

dNTP Mix PCR Grade 10 mM each

Pfu DNA Polymerase

One Step SYBR Green RT-PCR Mix

Hot start Taq DNA Polymerase

EntiLink PCR Master Mix

Additional information

Size

48T, 96T, 96T,48T

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