Name: Human ET-1-Endothelin1 ELISA Kit - Gencyst
SKU: ELK2444
Availability: InStock

Human ET-1-Endothelin1 ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

SKU: ELK2444 Category: ELISA Kit Tag: ELISA Kit

Overview of Human ET-1-Endothelin1 ELISA Kit

Product name:	Human ET-1-Endothelin1 ELISA Kit
Reactivity:	Human
Alternative Names:	ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1
Assay Type:	Sandwich
Sensitivity:	2.71 pg/mL
Standard:	500 pg/mL
Detection Range:	7.82-500 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5 h
Research Area:	Endocrinology;Cardiovascular biology;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T