Human EG-VEGF(Endocrine Gland Derived Vascular Endothelial Growth Factor) ELISA Kit

Human EG-VEGF(Endocrine Gland Derived Vascular Endothelial Growth Factor) ELISA Kit

262.00364.00

Specification 96T, 48T

SKU: ELK1008 Category: Tag:

Overview of Human EG-VEGF(Endocrine Gland Derived Vascular Endothelial Growth Factor) ELISA Kit

Product name: Human EG-VEGF(Endocrine Gland Derived Vascular Endothelial Growth Factor) ELISA Kit
Reactivity: Human
Alternative Names: PK1; EGVEGF; PRK1, PROK1; Prokineticin 1; Black Mamba Toxin-Related Protein; Mambakine
Assay Type: Sandwich
Sensitivity: 6.5 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cytokine;Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EG-VEGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EG-VEGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EG-VEGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EG-VEGF in the samples is then determined by comparing the OD of the samples to the standard curve.

 

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Size

48T, 96T, 96T,48T

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