Human FGF1(Fibroblast Growth Factor 1, Acidic) ELISA Kit

Human FGF1(Fibroblast Growth Factor 1, Acidic) ELISA Kit

262.00364.00

Specification 96T, 48T

SKU: ELK1019 Category: Tag:

Overview of Human FGF1(Fibroblast Growth Factor 1, Acidic) ELISA Kit

Product name: Human FGF1(Fibroblast Growth Factor 1, Acidic) ELISA Kit
Reactivity: Human
Alternative Names: ECGF; AFGF; ECGFA; ECGFB; FGF-Alpha; FGFA; HBGF1; ECGFB; HBGF1; Acidic Fibroblast Growth Factor; heparin-binding growth factor 1; Endothelial Cell Growth Factor, Beta
Assay Type: Sandwich
Sensitivity: 6.5 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cytokine;Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FGF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FGF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FGF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FGF1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Additional information

Size

48T, 96T, 96T,48T

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