Name: Human GPC4-Glypican 4 ELISA Kit - Gencyst
SKU: ELK1965
Availability: InStock

Human GPC4-Glypican 4 ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

SKU: ELK1965 Category: ELISA Kit Tag: ELISA Kit

Overview of Human GPC4-Glypican 4 ELISA Kit

Product name:	Human GPC4-Glypican 4 ELISA Kit
Reactivity:	Human
Alternative Names:	K-Glypican; Glypican Proteoglycan 4; Secreted glypican-4
Assay Type:	Sandwich
Sensitivity:	13.3 pg/mL
Standard:	2000 pg/mL
Detection Range:	31.25-2000 pg/mL
Sample Type:	serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5 h
Research Area:	Tumor immunity;Developmental science;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GPC4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GPC4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GPC4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GPC4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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