Name: Human HSP40-Heat Shock Protein 40 ELISA Kit - Gencyst
SKU: ELK1491
Availability: InStock

Human HSP40-Heat Shock Protein 40 ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

Clear

SKU: ELK1491 Category: ELISA Kit Tag: ELISA Kit

Overview of Human HSP40-Heat Shock Protein 40 ELISA Kit

Product name:	Human HSP40-Heat Shock Protein 40 ELISA Kit
Reactivity:	Human
Alternative Names:	DnaJ; DNAJB1; Sis1; RSPH16B; HSPF1; DnaJ homolog subfamily B member 1; Radial Spoke 16 Homolog B; Heat Shock 40kDa Protein 1; DnaJ protein homolog 1
Assay Type:	Sandwich
Sensitivity:	0.054 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5 h
Research Area:	Signal transduction;Developmental science;
	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HSP40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HSP40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HSP40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HSP40 in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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