Human IFNb(Interferon Beta) ELISA Kit

Human IFNb(Interferon Beta) ELISA Kit

262.00364.00

Specification 96T, 48T

SKU: ELK1101 Category: Tag:

Overview of Human IFNb(Interferon Beta) ELISA Kit

Product name: Human IFNb(Interferon Beta) ELISA Kit
Reactivity: Human
Alternative Names: IFNB1; IFN-B; IFB; IFF; IFNB; Interferon Beta 1 Fibroblast; IFN Beta
Assay Type: Sandwich
Sensitivity: 2.8 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cytokine;Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFNb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IFNb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IFNb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IFNb in the samples is then determined by comparing the OD of the samples to the standard curve.

 

Read More Description

Additional information

Size

48T, 96T, 96T,48T