Human IgA(Immunoglobulin A) ELISA Kit

Human IgA(Immunoglobulin A) ELISA Kit

262.00

Specification 96T, 48T

SKU: ELK1255 Category: Tag:

Overview of Human IgA(Immunoglobulin A) ELISA Kit

Product name: Human IgA(Immunoglobulin A) ELISA Kit
Reactivity: Human
Alternative Names: Immunoglobulin A
Assay Type: Sandwich
Sensitivity: 0.07 μg/mL
Standard: 100 μg/mL
Detection Range: 1.57-100 μg/mL
Sample Type: serum, plasma, saliva and other biological fluids
Assay Length: 3.5 h
Research Area: Infection immunity;Immune molecule; Hematology; Pulmonology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IgA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IgA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Human IgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a colour change. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IgA in the samples is then determined by comparing the OD of the samples to the standard curve.

 

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Size

48T, 96T, 96T,48T

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