Overview of Human KLK10-Kallikrein 10 ELISA Kit
| Product name: | Human KLK10-Kallikrein 10 ELISA Kit | 
| Reactivity: | Human | 
| Alternative Names: | NES1; PRSSL1; Kallikrein-Related Peptidase 10; Normal epithelial cell-specific 1; Protease serine-like 1 | 
| Assay Type: | Competitive Inhibition | 
| Sensitivity: | 48.3 pg/mL | 
| Standard: | 10000 pg/mL | 
| Detection Range: | 156.25-10000 pg/mL | 
| Sample Type: | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids | 
| Assay Length: | 2 h | 
| Research Area: | Enzyme & Kinase;Tumor immunity;Reproductive science; | 
| Test principle: | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KLK10 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK10 in the samples is then determined by comparing the OD of the samples to the standard curve. | 
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