Human KLK3(Kallikrein 3) ELISA Kit

Human KLK3(Kallikrein 3) ELISA Kit


Specification 96T, 48T

SKU: ELK1197 Category: Tag:

Overview of Human KLK3(Kallikrein 3) ELISA Kit

Product name: Human KLK3(Kallikrein 3) ELISA Kit
Reactivity: Human
Alternative Names: APS; KLK2A1; PSA; HK3; KLKB1; Seminin; Prostate Specific Antigen; Kallikrein-Related Peptidase 3; Semenogelase, g-Seminoprotein; P-30 Antigen; Plasma kallikrein
Assay Type: Sandwich
Sensitivity: 0.08 ng/mL
Standard: 12.5 ng/mL
Detection Range: 0.2-12.5 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Tumor immunity;Reproductive science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KLK3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Human KLK3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a colour change. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK3 in the samples is then determined by comparing the OD of the samples to the standard curve.

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48T, 96T, 96T,48T

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