Name: Human LDHB(Lactate Dehydrogenase B) ELISA Kit - Gencyst
SKU: ELK2155
Availability: InStock

Human LDHB(Lactate Dehydrogenase B) ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

Clear

SKU: ELK2155 Category: ELISA Kit Tag: ELISA Kit

Overview of Human LDHB(Lactate Dehydrogenase B) ELISA Kit

Product name:	Human LDHB(Lactate Dehydrogenase B) ELISA Kit
Reactivity:	Human
Alternative Names:	LDH-B; LDH-H; Renal carcinoma antigen NY-REN-46
Assay Type:	Sandwich
Sensitivity:	0.133 ng/mL
Standard:	20 pg/mL
Detection Range:	0.32-20 ng/mL
Sample Type:	Serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5 h
Research Area:	Enzyme & Kinase;Cardiovascular biology;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LDHB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LDHB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LDHB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LDHB in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T, 96T,48T

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