Human LFA1a-Lymphocyte Function Associated Antigen 1 Alpha ELISA Kit

Human LFA1a-Lymphocyte Function Associated Antigen 1 Alpha ELISA Kit

Specification 96T, 48T

SKU: ELK1774 Category: Tag:

Overview of Human LFA1a-Lymphocyte Function Associated Antigen 1 Alpha ELISA Kit

Product name: Human LFA1a-Lymphocyte Function Associated Antigen 1 Alpha ELISA Kit
Reactivity: Human
Alternative Names: CD11a; ITG-AL; LFA-1; LFA1-A; ITGAL; Integrin,Alpha L; CD11 antigen-like family member A; Leukocyte function-associated molecule 1 alpha chain
Assay Type: Sandwich
Sensitivity: 0.055 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5 h
Research Area: Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LFA1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LFA1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LFA1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LFA1a in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

dNTP Mix PCR Grade 10 mM each

Pfu DNA Polymerase

One Step SYBR Green RT-PCR Mix

Hot start Taq DNA Polymerase

EntiLink PCR Master Mix

Additional information

Size

48T, 96T

Reviews

There are no reviews yet.

Be the first to review “Human LFA1a-Lymphocyte Function Associated Antigen 1 Alpha ELISA Kit”

Your email address will not be published. Required fields are marked *

Filter by price