Human LIF(Leukemia Inhibitory Factor) ELISA Kit

Human LIF(Leukemia Inhibitory Factor) ELISA Kit


Specification 96T, 48T

SKU: ELK1162 Category: Tag:

Overview of Human LIF(Leukemia Inhibitory Factor) ELISA Kit

Product name: Human LIF(Leukemia Inhibitory Factor) ELISA Kit
Reactivity: Human
Alternative Names: CDF; D-FACTOR; HILDA; MLPLI; Cholinergic Differentiation Factor; Differentiation-Stimulating Factor; Melanoma-Derived LPL Inhibitor; Emfilermin
Assay Type: Sandwich
Sensitivity: 47 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cytokine;Tumor immunity;Infection immunity;Neuro science;Bone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LIF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LIF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Human LIF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a colour change. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LIF in the samples is then determined by comparing the OD of the samples to the standard curve.


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48T, 96T, 96T,48T

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