Name: Human MIF-Macrophage Migration Inhibitory Factor ELISA Kit - Gencyst
SKU: ELK1506
Availability: InStock

Human MIF-Macrophage Migration Inhibitory Factor ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

SKU: ELK1506 Category: ELISA Kit Tag: ELISA Kit

Overview of Human MIF-Macrophage Migration Inhibitory Factor ELISA Kit

Product name:	Human MIF-Macrophage Migration Inhibitory Factor ELISA Kit
Reactivity:	Human
Alternative Names:	GIF; GLIF; MMIF; Glycosylation-Inhibiting Factor; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase
Assay Type:	Sandwich
Sensitivity:	0.128 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5 h
Research Area:	Cytokine;Infection immunity;Rheumatology;Autoimmunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MIF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MIF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MIF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MIF in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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