Human MMP9(Matrix Metalloproteinase 9) ELISA Kit

Human MMP9(Matrix Metalloproteinase 9) ELISA Kit

262.00

Specification 96T, 48T

SKU: ELK1262 Category: Tag:

Overview of Human MMP9(Matrix Metalloproteinase 9) ELISA Kit

Product name: Human MMP9(Matrix Metalloproteinase 9) ELISA Kit
Reactivity: Human
Alternative Names: GELB; Gelatinase B; CLG4B; CLG4-B; 92 KDa Gelatinase; 92kDa Type IV Collagenase
Assay Type: Sandwich
Sensitivity: 0.059 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Enzyme & Kinase;Tumor immunity;Infection immunity;Cardiovascular biology;Hepatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MMP9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MMP9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Human MMP9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a color change. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MMP9 in the samples is then determined by comparing the OD of the samples to the standard curve.

 

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Size

48T, 96T, 96T,48T