Name: Human NFκB-p65-Nuclear Factor Kappa B p65 ELISA Kit - Gencyst
SKU: ELK1692
Availability: InStock

Human NFκB-p65-Nuclear Factor Kappa B p65 ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

SKU: ELK1692 Category: ELISA Kit Tag: ELISA Kit

Overview of Human NFκB-p65-Nuclear Factor Kappa B p65 ELISA Kit

Product name:	Human NFκB-p65-Nuclear Factor Kappa B p65 ELISA Kit
Reactivity:	Human
Alternative Names:	NFKB3; p65; REL-A; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3; Transcription Factor P65; Nuclear factor NF-kappa-B p65 subunit; RELA; V-Rel Reticuloendotheliosis Viral Oncogene Homolog A
Assay Type:	Sandwich
Sensitivity:	0.055 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5 h
Research Area:	Apoptosis;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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