Human NFκB-p65-Nuclear Factor Kappa B p65 ELISA Kit
Human NFκB-p65-Nuclear Factor Kappa B p65 ELISA Kit
€318.00 – €455.00
Specification 96T, 48T
Overview of Human NFκB-p65-Nuclear Factor Kappa B p65 ELISA Kit
| Product name: |
Human NFκB-p65-Nuclear Factor Kappa B p65 ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | NFKB3; p65; REL-A; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3; Transcription Factor P65; Nuclear factor NF-kappa-B p65 subunit; RELA; V-Rel Reticuloendotheliosis Viral Oncogene Homolog A |
| Assay Type: | Sandwich |
| Sensitivity: | 0.055 ng/mL |
| Standard: | 10 ng/mL |
| Detection Range: | 0.16-10 ng/mL |
| Sample Type: | tissue homogenates, cell lysates and other biological fluids |
| Assay Length: | 3.5 h |
| Research Area: | Apoptosis; |
| Test Principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
| Size | 48T, 96T |
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