Name: Human NF-κBp105-Nuclear factor NF-kappa-B p105 subunit ELISA Kit - Gencyst
SKU: ELK2228
Availability: InStock

Human NF-κBp105-Nuclear factor NF-kappa-B p105 subunit ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

Clear

SKU: ELK2228 Category: ELISA Kit Tag: ELISA Kit

Overview of Human NF-κBp105-Nuclear factor NF-kappa-B p105 subunit ELISA Kit

Product name:	Human NF-κBp105-Nuclear factor NF-kappa-B p105 subunit ELISA Kit
Reactivity:	Human
Alternative Names:	NF-KB1; EBP-1; KBF1; NF-Kappa-B; NFKB-P105; NFKB-P50; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear factor NF-kappa-B p105 subunit; NFkB; Nuclear Factor Kappa B
Assay Type:	Sandwich
Sensitivity:	0.056 ng/mL
Standard:	10 pg/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5 h
Research Area:	Signal transduction;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NF-κBp105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NF-κBp105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NF-κBp105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NF-κBp105 in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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