Human NSE-Enolase, Neuron Specific ELISA Kit
Overview of Human NSE-Enolase, Neuron Specific ELISA Kit
Product name: | Human NSE-Enolase, Neuron Specific ELISA Kit |
Reactivity: | Human |
Alternative Names: | ENO2; Enolase 2; Gamma Enolase; 2-phospho-D-glycerate hydro-lyase; Neural enolase |
Assay Type: | Sandwich |
Sensitivity: | 0.231 ng/mL |
Standard: | 40 pg/mL |
Detection Range: | 0.63-40 ng/mL |
Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Assay Length: | 3.5 h |
Research Area: | Enzyme & Kinase;Tumor immunity;Infection immunity;Neuro science; |
Test Principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NSE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NSE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NSE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NSE in the samples is then determined by comparing the OD of the samples to the standard curve. |
Read More Description
Additional information
Size | 48T, 96T |
---|