Overview of Human NT-Neurotensin ELISA Kit
| Product name: |
Human NT-Neurotensin ELISA Kit
|
| Reactivity: |
Human |
| Alternative Names: |
NTS; NTS1; NmN-125; NN; Tail peptide Neuromedin N |
| Assay Type: |
Competitive Inhibition |
| Sensitivity: |
14.1 pg/mL |
| Standard: |
3000 pg/mL |
| Detection Range: |
46.88-3000 pg/mL |
| Sample Type: |
serum, plasma and other biological fluids |
| Assay Length: |
2 h |
| Research Area: |
Reproductive science;Neuro science;Hormone metabolism; |
| Test Principle: |
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT in the samples is then determined by comparing the OD of the samples to the standard curve. |
dNTP Mix PCR Grade 10 mM each
Pfu DNA Polymerase
One Step SYBR Green RT-PCR Mix
Hot start Taq DNA Polymerase
EntiLink PCR Master Mix
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