Human NT-Neurotensin ELISA Kit
Overview of Human NT-Neurotensin ELISA Kit
Product name: |
Human NT-Neurotensin ELISA Kit |
Reactivity: | Human |
Alternative Names: | NTS; NTS1; NmN-125; NN; Tail peptide Neuromedin N |
Assay Type: | Competitive Inhibition |
Sensitivity: | 14.1 pg/mL |
Standard: | 3000 pg/mL |
Detection Range: | 46.88-3000 pg/mL |
Sample Type: | serum, plasma and other biological fluids |
Assay Length: | 2 h |
Research Area: | Reproductive science;Neuro science;Hormone metabolism; |
Test Principle: | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
Size | 48T, 96T |
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