Human NT-ProBNP-N-Terminal Pro-Brain Natriuretic Peptide ELISA Kit
Human NT-ProBNP-N-Terminal Pro-Brain Natriuretic Peptide ELISA Kit
€262.00 – €374.00
Specification 96T, 48T
Overview of Human NT-ProBNP-N-Terminal Pro-Brain Natriuretic Peptide ELISA Kit
Product name: | Human NT-ProBNP-N-Terminal Pro-Brain Natriuretic Peptide ELISA Kit |
Reactivity: | Human |
Alternative Names: | NT-Pro-BNP; ; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-29); BNP-32; Gamma-brain natriuretic peptide; nppb |
Assay Type: | Sandwich |
Sensitivity: | 15 pg/mL |
Standard: | 2500 ng/mL |
Detection Range: | 39.07-2500 pg/mL |
Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Assay Length: | 3.5 h |
Research Area: | Endocrinology;Cardiovascular biology; |
Test principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve. |
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One Step SYBR Green RT-PCR Mix
Additional information
Size | 48T, 96T |
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