Name: Human ON-Osteonectin ELISA Kit - Gencyst
SKU: ELK1682
Availability: InStock

Human ON-Osteonectin ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

SKU: ELK1682 Category: ELISA Kit Tag: ELISA Kit

Overview of Human ON-Osteonectin ELISA Kit

Product name:	Human ON-Osteonectin ELISA Kit
Reactivity:	Human
Alternative Names:	SPARC; BM-40; Secreted Protein,Acidic,Cysteine-Rich; Basement-membrane protein 40; Secreted protein acidic and rich in cysteine
Assay Type:	Sandwitch
Sensitivity:	1.21 ng/mL
Standard:	200 ng/mL
Detection Range:	3.13-200 ng/mL
Sample Type:	serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5 h
Research Area:	Metabolic pathway;Tumor immunity;Infection immunity;Hepatology;Bone metabolism;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ON. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ON. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ON, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ON in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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