Name: Human PDHa-Pyruvate Dehydrogenase Alpha ELISA Kit - Gencyst
SKU: ELK1709
Availability: InStock

Human PDHa-Pyruvate Dehydrogenase Alpha ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

Clear

SKU: ELK1709 Category: ELISA Kit Tag: ELISA Kit

Overview of Human PDHa-Pyruvate Dehydrogenase Alpha ELISA Kit

Product name:	Human PDHa-Pyruvate Dehydrogenase Alpha ELISA Kit
Reactivity:	Human
Alternative Names:	PDHCE1A; PHE1A; PHE1-A; PDHA1; PDH-A; Pyruvate Dehydrogenase(lipoamide)Alpha 1; Pyruvate Dehydrogenase E1 Component Subunit Alpha,Aomatic Form,Mitochondrial
Assay Type:	Sandwich
Sensitivity:	0.063 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5 h
Research Area:	Enzyme & Kinase;Metabolic pathway;
	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDHa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PDHa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PDHa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PDHa in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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