Human POMC-Proopiomelanocortin ELISA Kit
Overview of Human POMC-Proopiomelanocortin ELISA Kit
Product name: |
Human POMC-Proopiomelanocortin ELISA Kit |
Reactivity: | Human |
Alternative Names: | POC; NPP; ProOMC; ACTH; Pro-OMC; Pro Opiomelanocortin; Corticotropin-lipotropin; Corticotropin; Adrenocorticotropic hormone |
Assay Type: | Competitive Inhibition |
Sensitivity: | 43.9 pg/mL |
Standard: | 10000 pg/mL |
Detection Range: | 156.25-10000 pg/mL |
Sample Type: | Serum, plasma and other biological fluids |
Assay Length: | 2 h |
Research Area: | Endocrinology; |
Test Principle: | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human POMC protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human POMC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human POMC in the samples is then determined by comparing the OD of the samples to the standard curve. |
Read More Description
Additional information
Size | 48T, 96T |
---|
Reviews
There are no reviews yet.