Human SCT-Secretin ELISA Kit

Human SCT-Secretin ELISA Kit

318.00455.00

Specification 96T, 48T

SKU: ELK1753 Category: Tag:

Overview of Human SCT-Secretin ELISA Kit

Product name: Human SCT-Secretin ELISA Kit
Reactivity: Human
Alternative Names: Secretin
Assay Type: Competitive Inhibition
Sensitivity: 7.14 pg/mL
Standard: 1500 ng/mL
Detection Range: 23.44-1500 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2 h
Research Area: Endocrinology;Hepatology;Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human SCT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SCT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SCT in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

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Additional information

Size

48T, 96T

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