Overview of Human Slit2-Slit Homolog 2 ELISA Kit
Product name: |
Human Slit2-Slit Homolog 2 ELISA Kit |
Reactivity: |
Human |
Alternative Names: |
SLIL3 |
Assay Type: |
Sandwich |
Sensitivity: |
0.59 ng/mL |
Standard: |
100 pg/mL |
Detection Range: |
1.57-100 ng/mL |
Sample Type: |
tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Assay Length: |
3.5 h |
Research Area: |
Signal transduction;Tumor immunity;Neuroscience; |
Test Principle: |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Slit2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Slit2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Human Slit2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a colour change. The addition of sulphuric acid solution terminates the enzyme-substrate reaction and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Slit2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
dNTP Mix PCR Grade 10 mM each
Pfu DNA Polymerase
One Step SYBR Green RT-PCR Mix
Hot start Taq DNA Polymerase
EntiLink PCR Master Mix
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