Name: Human Smad7-Mothers Against Decapentaplegic Homolog 7 ELISA Kit - Gencyst
SKU: ELK1841
Availability: InStock

Human Smad7-Mothers Against Decapentaplegic Homolog 7 ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

SKU: ELK1841 Category: ELISA Kit Tag: ELISA Kit

Overview of Human Smad7-Mothers Against Decapentaplegic Homolog 7 ELISA Kit

Product name:	Human Smad7-Mothers Against Decapentaplegic Homolog 7 ELISA Kit
Reactivity:	Human
Alternative Names:	MADH7; MADH8; Mothers against DPP homolog 8
Assay Type:	Sandwich
Sensitivity:	0.056 ng/mL
Standard:	10 pg/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5 h
Research Area:	Developmental science;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Smad7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Smad7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Smad7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Smad7 in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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