Human TF(Thymic Factor) ELISA Kit

Human TF(Thymic Factor) ELISA Kit

262.00

Specification 96T, 48T

SKU: ELK1247 Category: Tag:

Overview of Human TF(Thymic Factor) ELISA Kit

Product name: Human TF(Thymic Factor) ELISA Kit
Reactivity: Human
Alternative Names: CD142; FIII; F3; TFA; Thromboplastin; Coagulation Factor III
Assay Type: Sandwich
Sensitivity: 5.9 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: plasma, t
Assay Length: 3.5h
Research Area: Hematology;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Human TF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a colour change. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TF in the samples is then determined by comparing the OD of the samples to the standard curve.

 

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Size

48T, 96T, 96T,48T