Mouse ANG-Angiogenin ELISA Kit

Mouse ANG-Angiogenin ELISA Kit

318.00455.00

Specification 96T, 48T

SKU: ELK2470 Category: Tag:

Overview of Mouse ANG-Angiogenin ELISA Kit

Product name: Mouse ANG-Angiogenin ELISA Kit
Reactivity: Mouse
Alternative Names: RNASE5; Ribonuclease,RNase A Family 5
Assay Type: Sandwich
Sensitivity: 0.131 ng/mL
Standard: 20 pg/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5 h
Research Area: Tumor immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ANG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ANG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ANG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ANG in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

Additional information

Size

48T, 96T, 96T,48T

Filter by price