Mouse CC16-Clara Cell Protein 16 ELISA Kit

Mouse CC16-Clara Cell Protein 16 ELISA Kit

318.00455.00

Specification 96T, 48T

SKU: ELK1643 Category: Tag:

Overview of Mouse CC16-Clara Cell Protein 16 ELISA Kit

Product name:

Mouse CC16-Clara Cell Protein 16 ELISA Kit

Reactivity: Mouse
Alternative Names: SCGB1A1; CC10; CCSP; UGB; Uteroglobin; Urinary Protein 1; Clara Cell Secretory Protein; Secretoglobin Family 1A Member 1; Clara-Cell Specific 10-kD Protein
Assay Type: Sandwich
Sensitivity: 30 pg/mL
Standard: 5000 ng/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: serum, plasma, lung lavage fluid, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5 h
Research Area: Infection immunity;Cardiovascular biology;Pulmonology;
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CC16. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CC16. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CC16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CC16 in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

dNTP Mix PCR Grade 10 mM each

Pfu DNA Polymerase

One Step SYBR Green RT-PCR Mix

Hot start Taq DNA Polymerase

EntiLink PCR Master Mix

Additional information

Size

48T, 96T, 96T,48T

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