Overview of Mouse CCL3L1-Chemokine C-C-Motif Ligand 3 Like Protein 1 ELISA Kit
| Product name: |
Mouse CCL3L1-Chemokine C-C-Motif Ligand 3 Like Protein 1 ELISA Kit |
| Reactivity: |
Mouse |
| Alternative Names: |
CCL3-L1; LD78; SCYA3L; SCYA3L1; MIP1AP; MIP1-Alpha-P; Small Inducible Cytokine A3-Like 1; Macrophage Inflammatory Protein 1-Alpha-P; Tonsillar Lymphocyte LD78 Beta |
| Assay Type: |
Sandwich |
| Sensitivity: |
35 pg/mL |
| Standard: |
5000 ng/mL |
| Detection Range: |
78.13-5000 pg/mL |
| Sample Type: |
Serum, plasma and other biological fluids. |
| Assay Length: |
3.5 h |
| Research Area: |
Cytokine;Infection immunity;Immune molecule; |
| Test principle: |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CCL3L1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CCL3L1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CCL3L1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CCL3L1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
dNTP Mix PCR Grade 10 mM each
Pfu DNA Polymerase
One Step SYBR Green RT-PCR Mix
Hot start Taq DNA Polymerase
EntiLink PCR Master Mix
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