Mouse CKMB(Creatine Kinase MB Isoenzyme) ELISA Kit

Mouse CKMB(Creatine Kinase MB Isoenzyme) ELISA Kit

262.00

Specification 96T, 48T

SKU: ELK1286 Category: Tag:

Overview of Mouse CKMB(Creatine Kinase MB Isoenzyme) ELISA Kit

Product name: Mouse CKMB(Creatine Kinase MB Isoenzyme) ELISA Kit
Reactivity: Mouse
Alternative Names: CK2; CK-MB; Creatine Kinase,Muscle/Brain
Assay Type: Sandwich
Sensitivity: 0.29 ng/mL
Standard: 50 ng/mL
Detection Range: 0.79-50 ng/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Enzyme & Kinase;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CKMB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CKMB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Mouse CKMB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a colour change. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CKMB in the samples is then determined by comparing the OD of the samples to the standard curve.

 

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Size

48T, 96T, 96T,48T

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