Name: Mouse FAS-Factor Related Apoptosis ELISA Kit - Gencyst
SKU: ELK1016
Availability: InStock

Mouse FAS-Factor Related Apoptosis ELISA Kit

€262.00 – €364.00

Specification 96T, 48T

Size

Clear

SKU: ELK1016 Category: ELISA Kit Tag: ELISA Kit

Overview of Mouse FAS-Factor Related Apoptosis ELISA Kit

Product name:	Mouse FAS-Factor Related Apoptosis ELISA Kit
Reactivity:	Mouse
Alternative Names:	CD95; ALPS1A; ALPS1-A; APO1; APT1; FAS1; FASTM; TNFRSF6; Fas Receptor; TNF Receptor Superfamily Member 6; Tumor Necrosis Factor Receptor Superfamily Member 6
Assay Type:	Sandwich
Sensitivity:	10.2 pg/mL
Standard:	2000 pg/mL
Detection Range:	31.25-2000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Apoptosis;Tumor immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FAS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FAS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FAS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FAS in the samples is then determined by comparing the OD of the samples to the standard curve.