Mouse IFABP/FABP2-Intestinal Fatty Acid Binding Protein ELISA Kit
Mouse IFABP/FABP2-Intestinal Fatty Acid Binding Protein ELISA Kit
€367.00 – €523.00
Specification 96T, 48T
Overview of Mouse IFABP/FABP2-Intestinal Fatty Acid Binding Protein ELISA Kit
Product name: | Mouse IFABP/FABP2-Intestinal Fatty Acid Binding Protein ELISA Kit |
Reactivity: | Mouse |
Alternative Names: | FABPI; IFABP; I-FABP; Intestinal-type fatty acid-binding protein; FABP2; Fatty Acid Binding Protein 2, Intestinal |
Assay Type: | Sandwich |
Sensitivity: | 0.056 ng/mL |
Standard: | 10 ng/mL |
Detection Range: | 0.16-10 ng/mL |
Sample Type: | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Assay Length: | 3.5 h |
Research Area: | Metabolic pathway;Tumor immunity;Endocrinology;Cardiovascular biology; |
Test principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IFABP/FABP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IFABP/FABP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IFABP/FABP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IFABP/FABP2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
Size | 48T, 96T, 96T,48T |
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