Mouse ITaC-Interferon Inducible T-Cell Alpha Chemoattractant ELISA Kit

Mouse ITaC-Interferon Inducible T-Cell Alpha Chemoattractant ELISA Kit

262.00364.00

Specification 96T, 48T

SKU: ELK1345 Category: Tag:

Overview of Mouse ITaC-Interferon Inducible T-Cell Alpha Chemoattractant ELISA Kit

Product name: Mouse ITaC-Interferon Inducible T-Cell Alpha Chemoattractant ELISA Kit
Reactivity: Mouse
Alternative Names: CXCL11; I-TAC; IP9; SCYB11; SCYB9B; B-R1; Chemokine(C-X-C-Motif)ligand 11; Small Inducible Cytokine Subfamily B Member 11; Interferon gamma-inducible protein 9
Assay Type: Sandwich
Sensitivity: 12.4 pg/mL
Standard: 2000 ng/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5 h
Research Area: Cytokine;Signal transduction;Infection immunity;
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ITaC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ITaC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ITaC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ITaC in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

dNTP Mix PCR Grade 10 mM each

Pfu DNA Polymerase

One Step SYBR Green RT-PCR Mix

Hot start Taq DNA Polymerase

EntiLink PCR Master Mix

Additional information

Size

48T, 96T

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