Mouse LIPG-Lipase, Endothelial ELISA Kit
Mouse LIPG-Lipase, Endothelial ELISA Kit
€318.00 – €455.00
Specification 96T, 48T
Overview of Mouse LIPG-Lipase, Endothelial ELISA Kit
| Product name: | Mouse LIPG-Lipase, Endothelial ELISA Kit |
| Reactivity: | Mouse |
| Alternative Names: | EL; EDL; Endothelial Lipase; Endothelial cell-derived lipase |
| Assay Type: | Sandwich |
| Sensitivity: | 0.61 ng/mL |
| Standard: | 100 ng/mL |
| Detection Range: | 1.57-100 ng/mL |
| Sample Type: | Serum, plasma and other biological fluids. |
| Assay Length: | 3.5 h |
| Research Area: | Enzyme & Kinase;Metabolic pathway; |
| The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LIPG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LIPG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LIPG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LIPG in the samples is then determined by comparing the OD of the samples to the standard curve. |
Read More Description
Additional information
| Size | 48T, 96T |
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